how to segment the gray scale image from background
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If the purpose here is to do automatic semantic segmentation of many horse images, you will probably need to train a convolutional neural network to do it. For animal recognition, DeepLab-v3+ seems to have good track record, judging by,
Matlab does have tools for building and training DeepLab-v3+. This might be a good place to start reading,
Obviously, though, the pre-trained version of the network may not work straight out of the box on your specific image database, so you will probably need to retrain it (or use transfer learning) using many manually segmented images. Therefore, it is well worth getting familiar with the Image Segmenter app, even if it doesn't serve your immediate aims.
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