Fixing vertical pattern noise in an image

Hello,

Does anybody know a way to fix vertical pattern noise in an image? I am doing a 2D correction already (taking a bias stack, averaging into one image, subtracting that from sample image). The 2D correction helps a lot but there are still some very noticeable single pixel columns that are all about a count or two from what they should be. I have tried applying a small amount of noise to the image which helps, but takes away from detail in the sample that I am looking at in the image? Does anyone have any other ideas for post acquiring editing?

Thank you!

Jason

[Image Analyst]

Can you post your images, both before and after?

Answer 1

[Image Analyst]

Maybe you can go down each column, and if the pixel is one count (gray level) less than the pixel on each side, then add the average offset to that center column.