Counting objects in an image

Dear Researchers, I have a problem of counting objects in an image. Suppose I have the below image (below link)

http://s20.postimage.org/bah41jkml/test1.jpg

which i got after segmentation.

how can I count the small circles in the middle of the image. Kindly suggest

Subrajeet

Answer 1

[Image Analyst]

1. Binarize the image so that it's logical. binaryImage = grayImage > 128
2. Call binaryImage = imclearborder(binaryImage) to get rid of the single line around the perimeter.
3. Invert the image: binaryImage = ~binaryImage, so now black circles are white
4. Call binaryImage = imclearborder(binaryImage) to get rid of the large (now white) background
5. Call bwlabel: [labeledImage, numberOfCircles] = bwlabel(binaryImage). This gives you the count.
6. Call measurements = regionprops(labeledImage) if you want other measurements

See my Image Segmentation Tutorial in my File Exchange if you want an example.

[subrajeet]

@Image Analyst from core of my heart I want to thankyou. Really I could not do it from yesterday. But one one thing can you tell me using which image processing technique this was achieved.

Thanks a lot again.

[Image Analyst]

It's just basic, elementary image segmentation via intensity thresholding. Nothing fancy at all. See updated instructions above.

Answer 2

[Thomas]

You could use techniques shown in many MATLAB image processing demos.

[subrajeet]

Thomas U can see in my image the circular objects in the centre have immideate neighbors as white pixels. But again beyond white pixels I have black pixels. So i could not understand how can I have the access over the center circles. I desire if by some means I can assign the outer black pixels as 1 or make them white then I can count the circles. But I am unable to do that.

Answer 3

[SANAHA PATHAN]

i want to count number of silkworm eggs from this figure, i used L=Count(binaryfig) this command for counting number of objects ,this command works but i actually dont know what actually this command count,and result of this command is different every time

[Image Analyst]

SANAHA, take a look at this link and then if you still have questions, post a new Question (not here) and upload your code and image(s). We actually don't know what your Count() function does either so be sure that's included in the code you upload.

[anna]

hey I segmented blue nuclei from a blood smear image using k means color segmentation. how can i count the number of segmented blue nuclei. could u please help me with the code for counting [ cant use imfindcircle because the segmented nuclei are not exactly cirlce in shape ]

[Image Analyst]

See Steve's blog: http://blogs.mathworks.com/steve/2013/11/19/watershed-transform-question-from-tech-support/