How to extract rgb values from an image and convert them into concentration?

Input image, extract rgb values from the image and convert those rgb values to concentration.

MATLAB Image Processing

How to code this ?

[Guillaume]

Concentration of what?

If you have images of fluorescent dyes, then

• colour is not usually related to concentration. It's the intensity.
• without calibration it's impossible to convert intensity into concentration.

[Priyal Bagwe]

Yes. Calibration is done.

[Jan]

Please explain exactly which inputs you have. Do you have a table to correlate RGB values to concentrations? Then the type of how this table is stored matters.

What have you tried so far and which problems occur?

[Priyal Bagwe]

On image insertion, I want the rgb value to be extracted (entire image is of one color) and then, concentration to be determined. Yes, I have a table correlating the RGB values to concentration. How do I proceed?

[Image Analyst]

Attach your one-color (i.e., gray scale) image and your calibration table in a .mat file with the paper clip icon.

Answer 1

[Rahul punk]

%% try this

rgbImage = permute(rgbImage, [3,1,2])

serialValues = rgbImage(:)'