How to automatically calculate distance between two brighter points in an gray scale image?

In my coding task, I am having an image after thresholding step which is having 9 brighter regions. How may I find distances between these brighter regions automatically in matlab. Thanks in anticipation.

[Adam]

Do you mean the positional distance or the colour distance? If you mean the latter then there are any number of ways to calculate the distance between 2 colours.

This being the one I have started using recently.

Answer 1

[KSSV]

You should be having the pixel value of the spots. Use find to get the location of those pixles and then use pdist or pdist2.