Can i use the same port in the Arduino to read the serial port and after reading it giving order to the Arduino?

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I am using a camera for taking a photo of objects using the Arduino, After that i want to sending it to the Matlab for processing. Arduino UNO has one serial port, can i use this to upload camera code and after that sending pictures by the same port i used to upload the camera code to the Matlab so he can do the processing?

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