We want to port code generated by deploytool to many different OS. Do we need to run deploytool on every OS?

We have MATLAB software we'd like to share with non-MATLAB users. Packaging it into a standalone executable with deploytool sounds like a good way to do this. However, since

1. we don't know the OS of the users, and

2. deploytool only makes executables that can be run in the same OS they were made in

do we need to run deploytool in every potential OS?

If so, this sounds prohibitive. Is there anything that would make it easier? e.g. Is it backward compatible, meaning we could run deploytool once in the newest Mac/Windows/Linux OS and the executable would run in all older versions of the OS?