Missing axes after executing the guihandles command

Hi

I have 3 axes on my figure but can only see 2 of them when I execute the command:

handles = guihandles

see both commands and ouput below.

An axis is missing on the second command. What could be the problem?

% This command yeilds the first result
handles = guidata(handles.Microscope)
% The second command yeilds the second result
handles = guihandles

handles1 =

struct with fields:

figure1: [1×1 Figure]

pushbutton7: [1×1 UIControl]

RoiFIT: [1×1 UIControl]

text4: [1×1 UIControl]

text3: [1×1 UIControl]

slider3: [1×1 UIControl]

slider2: [1×1 UIControl]

popupmenu2: [1×1 UIControl]

OrientationTweak: [1×1 UIControl]

Button_process_rotated: [1×1 UIControl]

pushbutton2: [1×1 UIControl]

filename_box: [1×1 UIControl]

File: [1×1 Menu]

button_process: [1×1 UIControl]

box_rotation: [1×1 UIControl]

Microscope_Image: [1×1 Axes]

kuva_profile: [1×1 Axes]

kuva_original: [1×1 Axes]

Load: [1×1 Menu]

output: [1×1 Figure]

handles =

struct with fields:

figure1: [1×1 Figure]

pushbutton7: [1×1 UIControl]

RoiFIT: [1×1 UIControl]

text4: [1×1 UIControl]

text3: [1×1 UIControl]

slider3: [1×1 UIControl]

slider2: [1×1 UIControl]

popupmenu2: [1×1 UIControl]

OrientationTweak: [1×1 UIControl]

Button_process_rotated: [1×1 UIControl]

pushbutton2: [1×1 UIControl]

filename_box: [1×1 UIControl]

File: [1×1 Menu]

button_process: [1×1 UIControl]

box_rotation: [1×1 UIControl]

kuva_profile: [1×1 Axes]

kuva_original: [1×1 Axes]

Load: [1×1 Menu]

imrect: [1×1 Group]

patch: [1×1 Patch]

what ould be the possible error.