Doubts about image processing

Hello,

I am doing a research where i have to work with images from the corneal nerves, and they are really dark. These pictures where taken by a confocal microscopy. I am having difficulties to divide the pictures in blocks. I really need to do this because i want to apply the histogram to small regions and then do the segmentation. Can anyone help me?

Thank you for your time.

Daniel Carvalho

[Jan]

Could you explain the difficulties you have with any details? What exactly means "really dark" - are they completely black? What kind of images are you working with? Can you post a small example of the images and the code?

[Daniel]

the images that i am working is something like this http://www.iovs.org/content/48/5/2017/F1.small.gif.

I want to isolate the nerves (the white stripes). I am using filters like the median, the functions imadjust, adapthisteq, bwmorph, rangefilt...

I can't show you my code because it is really confusing.

Answer 1

[Sean de Wolski]

Perhaps Brett Shoelson's local Otsu utility would help you:

http://www.mathworks.com/matlabcentral/fileexchange/29764-thresholdlocally

Answer 2

[Tim Zaman]

I would recommend using photoshop (or equivalent) first to edit the image to a usable format, as photoshop in that matter is more user-friendly, though less scientific.