How to add thickness to 2D images?

Anyone can help?

actually, I am doing the 3D Volume image. However, I combined the 2D slices into 3D image and showed them with 3D viewer. there is space between each slice. I want to give every slice a thinkness and combine into 3D volume image without any spacing.

[Ben11]

You might want to interpolate values between successive frames using interp3. I've never tried it on microscopy images though so it's just an idea :)

[Oliver Woodford]

What 3D viewer are you using? Can you give an example image of the kind output rendering you want?

[tabw]

1)viewer3d

https://www.google.com.hk/search?q=3D+confocal+image&safe=active&source=lnms&tbm=isch&sa=X&ei=R7PXU92WB4qE8gW5moDQBQ&ved=0CAYQ_AUoAQ&biw=1366&bih=599#facrc=_&imgdii=yS1aSiHChHY30M%3A%3BW09UtaM5oKENYM%3ByS1aSiHChHY30M%3A&imgrc=yS1aSiHChHY30M%253A%3BH3NglE0ybYJeVM%3Bhttp%253A%252F%252Fwww.nikoninstruments.com%252Fvar%252Fezwebin_site%252Fstorage%252Fimages%252Fmedia%252Fgallery-items%252Fphotomicrographs%252Fa1r-mp%252Fdaphnia-specimen%252F169748-1-eng-GB%252FDaphnia-Specimen_gallerylarge.jpg%3Bhttp%253A%252F%252Fwww.nikoninstruments.com%252FProducts%252FMicroscope-Systems%252FConfocal-Microscopes%252FA1R-MP-Multiphoton-Confocal3%252F(gallery)%3B358%3B360

the final product I want like this kind of image..

Thanks