gradient

how to calculate gradient of multichannels?

[Jan]

What are mutlichannels?

Answer 1

[sreejini]

My project is chromosome segmentation using multichannel watershed transform.the first step is calculating multichannel gradient.how to find it?

[Image Analyst]

Again, what are multichannels?

[Jan]

We need something like: [1000 x 200] DOUBLE arrays, or an equivalent description of the data format. For the calculations it does not matter, if your are working on chromosomes or orbits of stars in the galaxy.

[sreejini]

i have a dataset of chromosomes.within that 5 images of same chromosomes taken in different wavelength.i need to find gardient of these images together.

Answer 2

[Jan]

If you implement an algorithm in MATLAB (or any other programming language), the meaning of the values does not matter: chromosomes, dollars or pixel colors - all are just numbers. Explaining the meaning of your value repeatedly wastes time.

We do not have any chance to know, how your "dataset" is represented. Even "image" is not clear enough: An "image" can be a [M x N x 3] DOUBLE RGB array, or a [M x N] UINT8 greyscale matrix, or it can be a print-out and you are searching for a method to scan this. If you need an answer, you have to provide the necessary details of the question. But you ignore our questions.

Let me guess, that you have a [M x N x 5] DOUBLE array containing the greyscale values for 5 different wavelength. Then a "gradient" can be calculated between the different channels, or it can be the direction of the largest or average change of intensity, or a lot of other values.

So please delete your new question and edit the original one. Add all necessary details about the available input and the wanted output, and remove all unneeded details.