Extracting time slices and putting them back together in 4d for ITK snap?

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Taking a 4d MRI image that has two subjects stacked by time slices. I have been able to recreate one 3d image of one specific time frame but cannot get them to be placed together for a 4d image. I am having problems with the make_nii and save_nii and am not quite sure how to get it to work.

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